In Vivo & In Vitro Evaluation of Zinc Oxide & Cobalt-Doped Zinc Oxide Nanoparticles for Lieshmanicidal Activity

Project Title

In Vivo & In Vitro Evaluation of Zinc Oxide & Cobalt-Doped Zinc Oxide Nanoparticles for Lieshmanicidal Activity

Project Area of Specialization Biomedical EngineeringProject Summary

Leishmaniasis is a vector-borne disease caused by Leishmania parasites. Leishmanial transmission occurs when a sand fly sucks blood from an infected individual (human or animal). The parasite transformation occurs as it changes from the amastigote stage to the promastigote stage, taking about 4–25 days. The disease results in the development of ulcers and also affects other bodily organs. Leishmaniasis exists in three major forms, namely as mucosal leishmaniasis (ML), cutaneous leishmaniasis (CL), and visceral leishmaniasis (VL).

Cutaneous leishmaniasis (CL) is endemic in the tropical and subtropical countries. Antileishmanial drugs that are traditionally used for treatment of CL are mainly toxic, ineffective for some parasite isolates, and mostly expensive. Previous studies showed that some metal and metal oxide nanoparticles have antimicrobial activity. There is little information on antileishmanial activity of nanoparticles. Thus, the purpose of this research is to study antileishmanial effects of these nano particles Zinc Oxide & Cobalt-Doped Zinc Oxide in BALB/c mice and to evaluate the effectiveness of compounds of interest in comparison to standard meglumine antimonial. In this research study we will focus on Cutaneous Leishmaniasis (CL) and test Zinc Oxide & Cobalt-Doped Zinc Oxide nanoparticles for their lieshmanicidal activity.

Project Objectives

The aim of this research is to determine the anti-leishmanial properties of topical Cobalt (Co-NPs) and Nickel (Ni-NPs) nanoparticles in-vitro and in-vivo models.

• To develop in vivo cutaneous leishmaniasis in BALB/c mice.
• To prepare topical preparation of topical Zinc Oxide & Cobalt-Doped Zinc Oxide nanoparticles.
• To assess the in vitro leishmanicidal activity of Zinc Oxide & Cobalt-Doped Zinc Oxide nanoparticles in comparison to the standard meglumine antimoniate
• To evaluate the in-vivo effect of Zinc Oxide & Cobalt-Doped Zinc Oxide nanoparticles on cutaneous leishmaniasis and on local pain score, edema, body weight and body temperature of L.tropica BALB/c mice.

Project Implementation Method

This project will be implemented in the following ways:

• The outcome will be published to spread awareness of the properties of Zinc Oxide & Cobalt-Doped Zinc Oxide nanoparticles on Leishminasis.
• The cure for Leishmaniasis could potentially be discovered through this research.
• The actual product will be available in the market in the form of a paste for patients to apply on the infected area.
• The development of new techniques to carry out this research. Potential for the publication of new techniques.

Benefits of the Project

Benifits of this research are as follows:

• Assessment of in vitro leishmanicidal activity of Zinc Oxide & Cobalt-Doped Zinc Oxide nanoparticles in comparison to the standard meglumine antimoniate.
• Evaluation of the in-vivo effect of Zinc Oxide & Cobalt-Doped Zinc Oxide nanoparticles on cutaneous leishmaniasis and on local pain score, edema, body weight and body temperature of L.tropica BALB/c mice.
• To potentially develop a cure for Leishminiasis.
• To evaluate the properties of of Zinc Oxide & Cobalt-Doped Zinc Oxide nanoparticles on Leishminasis.

Technical Details of Final Deliverable

Technical Details of Final Deliverable:

• To evaluate the in-vivo effect of Zinc Oxide & Cobalt-Doped Zinc Oxide nanoparticles on cutaneous leishmaniasis and on local pain score, edema, body weight and body temperature of L.tropica BALB/c mice.
• To assess the in vitro leishmanicidal activity of Zinc Oxide & Cobalt-Doped Zinc Oxide nanoparticles in comparison to the standard meglumine antimoniate.
• The potential product will be available in the market in the form of a paste for patients to apply on the infected area.

Final Deliverable of the Project Hardware SystemCore Industry MedicalOther Industries Education , Medical , Health Core Technology OthersOther Technologies OthersSustainable Development Goals Good Health and Well-Being for PeopleRequired Resources

Item Name	Type	No. of Units	Per Unit Cost (in Rs)	Total (in Rs)
			Total in (Rs)	63000
BALB/c Mice	Equipment	40	800	32000
Zinc Oxide Nanoparticles	Equipment	1	9000	9000
Cobalt-Doped Zinc Oxide Nanoparticles	Equipment	1	12000	12000
Mice Food	Miscellaneous	1	8000	8000
Stationary	Miscellaneous	1	2000	2000