Evaluating Bio-Anode For Bio energy Production Using Solar-Powered Microbial Electrolysis
First of all, we took an anode that is a graphite anode and a copper anode, then we place these anodes in separate beakers filled with slurry and water solution to grow bacteria community on the surface of the anode. Slurry has a large amount of organic material and bacteria etc, when the bacteria h
2025-06-28 16:27:07 - Adil Khan
Evaluating Bio-Anode For Bio energy Production Using Solar-Powered Microbial Electrolysis
Project Area of Specialization Electrical/Electronic EngineeringProject SummaryFirst of all, we took an anode that is a graphite anode and a copper anode, then we place these anodes in separate beakers filled with slurry and water solution to grow bacteria community on the surface of the anode. Slurry has a large amount of organic material and bacteria etc, when the bacteria have been grown on the anodes we took the anode sample and sent it in to the lab to confirm the bacterial community on the anode surface. After this, we place these anodes into two separate beakers filled with substrates. A small amount of DC through the solar cell is provided for the electrolysis, the bacteria that populate the wastewater oxidize the organic substrate and create various electrons (e-) and protons(H+) ions. The electrons present on the anode then proceed towards the cathode and combine with the protons and produce hydrogen and methane.
An electron microscope confirmed the presence of the bacterial community on the anode, as well as, on the cathode surface. Within 15 days, the bioenergy-hydrogen and methane [CH4] were produced using synthetic dairy compost wastewater (SDMW), potato starch wastewater, textile industry wastewater, and graphite, and copper anodes, with the growth of microbial networks on the anode. At last, we have the gas chromatography results to show how much hydrogen and methane is produced in our project
Project Objectives- manage slurry
- waste water treatment
- textile industry waste water treatment
- dairy manure treatment
- potatoes industry waste water treatment
- hydrogen generation
- current generation
- clean energy
- renewable energy
- sustainable growth
Project Implementation Method
In our project we performed experiment on three different materials to analyze the production of Hydrogen gas. First we add different percentage of slurry in different materials to analyze the production of hydrogen gas in different materials and then enter different materials in different jars. After this we pass Nitrogen gas after air tighting to make anerobic environment inside the beaker
Textile Wastewater
In this after 25 percent of slurry addition we took textile wastewater from Crescent textile industry and then we add graphite anode and stainless steel cathode. The community of microbes is already grow on both of them and then apply a voltage of 1.5v in it . after 20 days we analyze that the piston of our injection starts to move in opposite side which indicate us that gas is start to generate inside the cylinder and after analyze the production of gases on GC we came to know that 38 percent of hydrogen produced inside the jar.
Potato Starch Wastewater
In this after 25 percent of slurry addition we took 1 Kg of Potato for making chips and after peeling the potato we wash it with water for 10 to 15 minutes in a beaker and that water is added into beaker and then we add cooper anode and stainless steel cathode. The community of microbes is already grow on both of them and then apply a voltage of 1.5v in it . after 20 days we analyze that the piston of our injection starts to move in opposite side which indicate us that gas is start to generate inside the cylinder and after analyze the production of gases on GC we came to know that 26 percent of hydrogen produced inside the jar.
Dairy Manure Wastewater
In this after addition of 65 percent of slurry we added rest of water into beaker and then we add cooper anode and stainless steel cathode. The community of microbes is already grow on both of them and then apply a voltage of 1.5v in it . after 20 days we analyze that the piston of our injection starts to move in opposite side which indicate us that gas is start to generate inside the cylinder and after analyze the production of gases on GC we came to know that 5 percent of hydrogen produced inside the jar.
Benefits of the ProjectBenefits of the Project
Our project is actually to analyze the production of hydrogen gas through different materials .
These are the following benefits of Hydrogen gas
Waste Water treatment and hygdorgen production
Hydrogen Fuel cell produce Electricity:
Hydrogen fuel cells produce electricity by combining hydrogen and oxygen atoms. The hydrogen reacts with oxygen across an electrochemical cell similar to that of a battery to produce electricity, water, and small amounts of heat .Many different types of fuel cells are available for a wide range of applications. Small fuel cells can power laptop computers and even cell phones, and military applications. Large fuel cells can supply electricity to electric power grids, supply backup or emergency power in buildings, and supply electricity in places that are not connected to electric power grids
Hydrogen use in vehicles :
The interest in hydrogen as an alternative transportation fuel stems from its ability to power fuel cells in zero-emission vehicles (vehicles with no emissions of air pollutants), its potential for domestic production, and the fuel cell's potential for high efficiency. A fuel cell may be two to three times more efficient than an internal combustion engine running on gasoline. Hydrogen can also fuel internal combustion engines, but burning hydrogen results in nitrogen oxides emissions and is less efficient than use in fuel cells. Several vehicle manufacturers have light-duty hydrogen fuel cell vehicles available for lease or sale in California where there are public hydrogen fueling stations. Test vehicles are also available in limited numbers to organizations with access to hydrogen fueling stations
Technical Details of Final DeliverableThe maximum amount of hydrogen production rate (m3-h2/m3-day) and columbic efficiency (%) and the two most important variables for performance efficiency comparison for microbial electrolysis cells (MECs). The three main criteria that influence the MECs' presentation productivity are as follows:
I. Reactor configuration.
II. Electrode material.
III. Substrates.
We shall now hypothetically portray the influence of these factors on the hydrogen production rate and MEC columbic efficacy.
The two main types of reactor design are as follows:
- Two-chamber MEC.
- Single chamber MEC.
The functioning rule of two MECs is practically something very similar, nonetheless, is a little contrast in particle trade film between two terminals, two-chamber MEC gives greater virtue to h2. It keeps the back dissemination of hydrogen from cathode to anode

| Item Name | Type | No. of Units | Per Unit Cost (in Rs) | Total (in Rs) |
|---|---|---|---|---|
| Total in (Rs) | 48900 | |||
| beakers of different ml | Equipment | 4 | 400 | 1600 |
| anode | Equipment | 3 | 900 | 2700 |
| cathode | Equipment | 3 | 500 | 1500 |
| injections,tubes,silicon glue gun etch | Equipment | 10 | 200 | 2000 |
| nitrogen cyliner | Equipment | 1 | 3600 | 3600 |
| SEM analysis | Equipment | 2 | 3000 | 6000 |
| GC analysis | Equipment | 3 | 4000 | 12000 |
| glass box | Equipment | 1 | 1500 | 1500 |
| hot plate | Equipment | 1 | 4000 | 4000 |
| power supply | Equipment | 1 | 5000 | 5000 |
| small solar plate | Equipment | 1 | 1000 | 1000 |
| Travel to NUST UPCASE | Miscellaneous | 2 | 4000 | 8000 |