A Beta Cyclodextrin based Green composite for Wound Healing

Project Title

A Beta Cyclodextrin based Green composite for Wound Healing

Project Area of Specialization Wearables and ImplantableProject Summary

A infection is a physical harm to the body in which the skin is tear and is exposed to external environment by which the risk of fatal diseases is high in that area of the body, so for infection controlling Doctors prescribed oral antibiotics which have fatal side effect on the human health as all known, so the best option of replacing such orally antibiotics for wounds healing with the dressing composites which have better results and biocompatibility as compare to the orally antibiotic drugs. Preparation of such Scaffold composite as Green composite because it is purely composed of the natural material with high drug-releasing properties, using of  Beta Cyclodextrin as drug carriers, silk fibroins for dressing purpose and ciprofloxacin as an antimicrobial drug for wound healing.

In this study, silk fibroin and Beta Cyclodextrin composite scaffolds will be fabricated using a freeze-drying method for effective wound dressing and drug-releasing application. The prepared Beta Cyclodextrin and silk fibroin 3D scaffold will be loaded with ciprofloxacin  HCL which is a soluble antimicrobial drug and commonly used in tissue engineering. Beta Cyclodextrin, which also acts as a drug carrier may carry and release Ciprofloxacin HCL to the wound site for a certainly long duration of time. The prepared composite scaffolds will be characterized by Fourier Transform Infrared (FTIR) spectroscopy, Scanning Electron Microscopy (SEM), X-Ray Diffraction (XRD) and evaluated for antibacterial properties. These composite scaffolds using silk fibroin, Beta Cyclodextrin with an appropriate antimicrobial agent could successfully inhibit the growth of bacteria. Therefore, Green composite could be a promising candidate for wound healing applications.

A infection is a physical harm to the body in which the skin is tear and is exposed to external environment by which the risk of fatal diseases is high in that area of the body, so for infection controlling Doctors prescribed oral antibiotics which have fatal side effect on the human health as all known, so the best option of replacing such orally antibiotics for wounds healing with the dressing composites which have better results and biocompatibility as compare to the orally antibiotic drugs. Preparation of such Scaffold composite as Green composite because it is purely composed of the natural material with high drug-releasing properties, using of  Beta Cyclodextrin as drug carriers, silk fibroins for dressing purpose and ciprofloxacin as an antimicrobial drug for wound healing.

Project Objectives

1. Preparation of Cyclodextrin incorporated and Ciprofloxacin loaded antimicrobial composite for wound healing.
2. To evaluate antibacterial activity by Agar Diffusion test method.
3. Structural and surface characterization of prepared scaffolds using SEM, FTIR, and EDX method
4. Characterization and Comparison of Cyclodextrin integrate double and chitosan-based single carrier composite for wound healing.

Project Implementation Method

This project is divided into three stages. The first stage is to extract silk fibroin and eliminate sericin protein from Bombyx mori silk cocoons by degumming process and obtain silk fibroin solution by chemical treatment. The second stage requires the silk fibroin solution to be mixed with Beta Cyclodextrin using citric acid as a cross-linking agent to prepare a uniform mixture of Beta Cyclodextrin and silk fibroin. This mixture would be transferred to Petri dishes and frozen overnight. At last, the freeze-dried plates would be lyophilized for 24 hours and 3D composite scaffolds are obtained. The prepared silk fibroin composite scaffold would then be loaded with 0.5% Ciprofloxacin solution and dried at room temperature. Lastly, the yield composite product would be characterized using FTIR, SEM, and XRD and tested for its antibacterial activity.

Benefits of the Project

In recent research studies, there is one of the most serious challenges to prevent the infection by controlling drug delivery system. In the perspective of the clinical applications mostly the antibacterial drugs, antibiotics are given to the patients for infection prevention which is fatal for the patient health because the antibiotics and oral drugs have too much side effect as compared to the dressing drugs, but also dressing drugs facing problem in drug-releasing properties which is important for infection healing. However, this project provides a controlled and long durable antibiotic drug release system on the 3D composite scaffold to minimize infections in chronic wounds due to Beta Cyclodextrin. These composite scaffolds are expected to provide a slow antibiotic release to the wound site leading to rapid wound healing. Thus, these scaffolds will prevent the body from harmful side effects of antibiotics and low quality dressing drugs also solve the problem of the releasing drug for a certain duration of the time.

Technical Details of Final Deliverable

The project comprises the preparation of silk fibroin and Beta Cyclodextrin composite scaffolds. The prepared composite scaffolds for wound dressing will include surface and structural characterization using Fourier Transform Infrared (FTIR) spectroscopy, Scanning Electron Microscopy (SEM), and X-Ray Diffraction (XRD). Moreover, the prepared composite samples would be evaluated for its antibacterial properties using the agar diffusion test method, then Characterize and Compare of Cyclodextrin integrate double and chitosan-based single carrier composite for wound healing

Final Deliverable of the Project Hardware SystemCore Industry MedicalOther Industries Health Core Technology Wearables and ImplantablesOther TechnologiesSustainable Development Goals Good Health and Well-Being for PeopleRequired Resources

Elapsed time in (days or weeks or month or quarter) since start of the project	Milestone	Deliverable
Month 1	Search for research papers and gathering information related to the recent work.	Documentation
Month 2	Literature Review	Documentation and references
Month 3	Detailed study of main materials that are used i e Beta Cyclodextrin, Silk fibrons	Documentation and a clear idea on the requirements of the project.
Month 4	Extraction of silk fibroin from bombyx mori silk cocoons using degumming process	Performing experiment for removal of sericin protein by degumming.
Month 5	Formation of silk fibroin solution	Experiment for obtaining silk fibroin solution and documentation.
Month 6	Preparation of silk fibroin and Beta Cyclodextrin composite scaffold	Successful Experiments and documentation.
Month 7	Surface and structural characterization of Composite samples	Analysis and documentation
Month 8	Evaluation test for antibacterial activity	Analysis and documentation
Month 9	Characterize and compare the beta Cyclodextrin integrate double and chitosan based single carrier composite for wound healing.	Analysis and documentation
Month 10	Evaluate the results of both samples	Analysis and documentation
Month 11	Finalize the project	Complete project report with prepared composite scaffold