Synthesis and Characterization of Green Silver Nanoparticles using Aloe Vera as Stabilizing agent and investigation of antimicrobial properties

In recent years, nanotechnology is considered as one of the enticing fields in science for the researchers. Nanotechnology administers nanoparticles of size ranging from 1-100 nm. There is a tremendous use of nanoparticles due to their miniature size, orientation and substantial properties. By means

Project Title

Synthesis and Characterization of Green Silver Nanoparticles using Aloe Vera as Stabilizing agent and investigation of antimicrobial properties

Project Area of Specialization

Biomedical Engineering

Project Summary

In recent years, nanotechnology is considered as one of the enticing fields in science for the researchers. Nanotechnology administers nanoparticles of size ranging from 1-100 nm. There is a tremendous use of nanoparticles due to their miniature size, orientation and substantial properties. By means of different physical, chemical, and biological approaches, these petite particles can easily be prepared. However, the biological approach is majorly an emerging technique for preparation of nanoparticles, because this method is much less complicated in comparison with other approaches because it is much less time consuming, and more importantly eco-friendly. Green synthesis of nanoparticles was carried out by mixing an aqueous solution of leaf extract of Aloe barbadensis miller (aloe vera) and silver nitrate AgNO3 solution.Silver has particular significance to this procedure due to its evocative chemical and physical  properties. Plant extract and metal ion solutions were mixed together in a fixed ratio and after that color change in the solution was observed, this change in color signified that nanoparticles  are formed. The nanoparticles were characterized by using instruments that includes UV-vis spectrophotometer and scanning Electron Microscope (SEM). To validate the activities of the synthesized nanoparticles, different biological assays will be analyzed that comprises antibacterial, antioxidant and cytotoxic assays. Silver and aloe vera both already considered as Antibacterial agents so synergistic antimicrobial activity is expected.

Project Objectives

The objectives are :

Green Synthesis of Silver Nanoparticles from AloeVera plant
 To check the anti-bacterial activity of synthsized nanoparticles.

Project Implementation Method

PREPARATION OF ALOE VERA LEAVE EXTRACT

PREPARATION OF SILVER NANOPARTICLES (Ag NP) BY BIO EXTRACT

HYDROTHERMAL TREATMENT ON SILVER NANOPARTICLES

CHARACTERIZATION THROUGH ELECTRON MICROSCOPY

CHARACTERIZATION THROUGH SPECTROPHOTOMETERY

ANTIBACTERIAL ACTIVITY

Benefits of the Project

TO DISCOVER THE SYNERGISTIC EFFECT OF SILVER AND ALOE VERA GEL AS ANTIBACTERIAL AGENTS.

Technical Details of Final Deliverable

THE FINAL PRODUCT WITH BE THE NANOPARTICLES IN POWDERED FORM.

Final Deliverable of the Project

HW/SW integrated system

Core Industry

Medical

Other Industries

Agriculture , Food , Health

Core Technology

Wearables and Implantables

Other Technologies

Sustainable Development Goals

Good Health and Well-Being for People

Required Resources

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