Designing a portable device to measure protein concentration

Project Title

Designing a portable device to measure protein concentration

Project Area of Specialization Biomedical EngineeringProject Summary

Measurement of the total protein concentration is also a crucial step in biochemistry and standard clinical laboratory procedures in many applications.

It is also important to accurately qualify protein in the sample before starting any protein Form of protein analysis, particularly comparative techniques. Different methods will be used to check and extend the same milk and raw egg sample items for determining the efficiency of the overall protein estimation data.

 In this project, the biuret test, the qualitative analysis test for protein is extended to quantitative analysis of proteins by determining the concentration of protein in given sample.

 The main sensing element for this project is Colour sensor TCS3200. The main objective of this project is to estimation of protein concentration in given sample of milk by exploiting the relation between spectral absorptivity and the concentration of protein. We propose to use optical sensor to accurately estimate the concentration of protein in different types of milk. The research objectives are accuracy, response time, portability, and sample size.

We intend to design and fabricate a prototype of a spectroscopic device that can determine the concentration of protein within 5 minutes with an accuracy of around 90% in a sample size not greater than 5ml collected from a dairy outlet.

Project Objectives

Our objectives are:

• To prepare and analyze sample solution.
• Analyze and determine results of sample solution.
• To design a portable device for the estimation of protein concentration.
• To compare our results with multiple laboratory reports and research work.
• To analyze the effect of the presence of other nutrients on the absorptivity of the sample under consideration.

Project Implementation Method

We plan to design a portable device to measure protein concentration in food items.

The project implementation methods are based on:

Step no.1

To prepare sample solution of milk and raw egg by using copper sulphate as reagent and sodium hydro oxide as alkaline base.

This method is used to determine if the sample contains protein.

The presence of protein can be determined by observing the purple/violet colour.

This method is known as biuret test method.

We also prepare solution by centrifugation method.

Step no.2

Designing a device

To make circuit by interfacing of Arduino uno and RGB color sensor.

The white LED is connected in series with the circuit and sample.

The result will be shown in display.

Benefits of the Project

Some of the benefits that can be achieved from this project are:

• Easy to handle
• Portable
• The set-up is less time consuming
•  with a small sample size that is less than 5ml.

Technical Details of Final Deliverable

At the end of this project, we expect to have a proper device that can determine the protein concentration in a small sample size, with less time and high accuracy.

Final Deliverable of the Project Hardware SystemCore Industry FoodOther Industries Health Core Technology OthersOther TechnologiesSustainable Development Goals Good Health and Well-Being for People, Decent Work and Economic Growth, Industry, Innovation and InfrastructureRequired Resources

Item Name	Type	No. of Units	Per Unit Cost (in Rs)	Total (in Rs)
			Total in (Rs)	80000
Arduino MKR1000	Equipment	2	10000	20000
LCD display	Equipment	1	1000	1000
TCS RGB color sensor	Equipment	2	1500	3000
Copper sulfate	Equipment	3	3500	10500
Sodium hydroxide	Equipment	2	2000	4000
Acetic Acid	Equipment	1	1500	1500
Quartz Cuvette/Glass wares	Equipment	1	30000	30000
Transportation/Print	Miscellaneous	1	10000	10000